Fig. 5. STAT3 knockdown augments diallyl trisulfide (DATS)-mediated inhibition of leptin-stimulated cell invasion in MDA-MB-231 cells. (A) Immunoblotting for STAT3 and β-actin in MDA-MB-231 cells transfected with control or STAT3-targeted siRNA. (B) Representative H & E-stained images (× 100 magnification) and quantification of cell invasion in 16-hour serum-starved transiently transfected MDA-MB-231 cells with control or STAT3 siRNA and treated for 24 hours with dimethyl sulfoxide (control) or 40 µmol/L DATS in the absence or presence of 100 ng/mL leptin. Results are shown as mean ± SD (n = 3) and statistical analysis was performed by one-way ANOVA followed by Bonferroni’s multiple comparisons test (*P < 0.05). Experiment was repeated twice with consistent results.

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