Journal of Cancer Prevention

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Fig. 4.

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Fig. 4. Diallyl trisulfide (DATS) treatment inhibits leptin-stimulated migration and invasion of MDA-MB-231 cells. (A) Representative H & E-stained images (× 100 magnification) showing cell migration and its quantification. Cells were serum starved for 16 hours and then treated with dimethyl sulfoxide (DMSO; control) or 40 µmol/L DATS for 24 hours in the absence or presence of 100 ng/mL leptin. Results are shown as mean ± SD (n = 3) and statistical analysis was performed by one-way ANOVA followed by Bonferroni’s multiple comparisons test (*P < 0.05). Experiment was repeated thrice with consistent results. (B) Representative H & E-stained images (× 100 magnification) showing cell invasion and its quantification. Cells were serum starved for 16 hours and then treated with DMSO (control) or 40 µmol/L DATS for 24 hours in the absence or presence of 100 ng/mL leptin. Results are shown as mean ± SD (n = 2-3) and statistical analysis was performed by one-way ANOVA followed by Bonferroni’s multiple comparisons test (*P < 0.05). Experiment was repeated thrice with consistent results. (C) Reverse transcription-PCR analysis for VEGF, matrix metalloproteinase MMP-2, MMP-9, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) using lysates from 16-hour serum starved MDA-MB-231 cells treated with DMSO (control) or 40 µmol/L DATS for 2 hours and then further incubated for 22 hours after adding 100 ng/mL leptin. Numbers above bands indicate change in expression relative to solvent control treated cells. Experiment was repeated thrice with consistent results.
Journal of Cancer Prevention 2020;25:1~12 https://doi.org/10.15430/JCP.2020.25.1.1
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