Journal of Cancer Prevention

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Fig. 3.

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Fig. 3. Diallyl trisulfide (DATS) treatment inhibits basal as well as leptin-stimulated expression of pro-survival proteins regulated by STAT3. (A) Immunoblotting for Bcl-2, Bcl-xL, Cyclin D1, and β-actin using lysates from 16-hour serum starved MCF-7 (left panel) and MDA-MB-231 (right panel) cells treated with dimethyl sulfoxide (DMSO; control) or 40 µmol/L DATS for 2 hours and then further incubated for 22 hours after adding 100 ng/mL leptin. Experiment was repeated at least twice. (B) Reverse transcription-PCR analysis for Bcl-2, Bcl-xL, Cyclin D1, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) using 16-hour serum starved MCF-7 (left panel) and MDA-MB-231 (right panel) cells treated with DMSO (control) or 40 µmol/L DATS for 2 hours and then further incubated for 22 hours after adding 100 ng/mL leptin. Numbers above bands indicate change in expression relative to solvent control treated cells. Experiment was repeated at least twice.
Journal of Cancer Prevention 2020;25:1~12 https://doi.org/10.15430/JCP.2020.25.1.1
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