Journal of Cancer Prevention

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pISSN 2288-3649
Fig. 2.

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Fig. 2. Diallyl trisulfide (DATS) treatment inhibits basal as well as leptin-induced cell growth in human breast cancer cells. (A) Quantitation of cell viability of 16-hour serum-starved MCF-7 (left panel) and MDA-MB-231 (right panel) cells treated with dimethyl sulfoxide (DMSO; control) or 40 µmol/L DATS for 2 hours and then further incubated for 22 hours after adding 100 ng/mL leptin. Results shown are mean ± SD (n = 3) and statistical analysis was performed by one-way ANOVA followed by Bonferroni’s multiple comparisons test (*P < 0.05). Experiment was repeated twice with consistent results. (B) Representative images of colonies from 16-hour serum-starved MCF-7 (upper panel) and MDA-MB-231 (lower panel) cells treated with DMSO (control) or 40 µmol/L DATS for 2 hours and then further incubated for 12 days after adding 100 ng/mL leptin. Media containing agents was changed every third day. (C) Quantitation of colony formation from data shown in panel B. Results shown are mean ± SD (n = 3) and statistical analysis was performed by one-way ANOVA followed by Bonferroni’s multiple comparisons test (*P < 0.05). Experiment was repeated twice with consistent results.
Journal of Cancer Prevention 2020;25:1~12 https://doi.org/10.15430/JCP.2020.25.1.1
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