Journal of Cancer Prevention

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Fig. 1.

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Fig. 1. Diallyl trisulfide (DATS) treatment inhibits constitutive as well as leptin-induced STAT3 phosphorylation at Tyr-705 in human breast cancer cells. (A) Immunoblotting for phospho (P)-STAT3, STAT3, and β-actin using lysates from 16-hour serum starved MCF-7 (left panel) and MDA-MB-231 (right panel) cells. Cells were first serum starved for 16 hours and then exposed to dimethyl sulfoxide (DMSO; control) or 40 µmol/L DATS for 2 hours and then further treated for 30 minutes or 1 hour after adding 100 ng/mL leptin. Experiment was repeated twice. (B) Immunocytochemistry for P-STAT3 (red fluorescence) in MCF-7 (left panel) and MDA-MB-231 (right panel) cells. Cells were serum starved for 16 hours and then treated with DMSO (control) or 40 µmol/L DATS for 2 hours and further incubated for 3 hours after adding 100 ng/mL leptin. SYTOX Green was used to stain nucleus. Images were captured at 100 × objective magnification. Experiment was repeated twice with comparable results.
Journal of Cancer Prevention 2020;25:1~12 https://doi.org/10.15430/JCP.2020.25.1.1
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