Fig. 1. Elevated Akt phosphorylation and GPR30 expression in BRCA1 deficient triple negative breast cancer cells. (A) Phosphorylation of Akt and GPR30 expression were compared with respect to the BRCA1 status in MDA-MB-231 and HCC1937 cells by Western blot analysis. (B) The levels of GPR30 mRNA were measured by reverse transcription-PCR. (C) MDA-MB-231 cells were transfected with BRCA1-siRNA, and then expression of BRCA1 and GPR30 was determined by immunoblot analysis. (D) HCC1937 cells are reverse transfected with 20 nM GPR30-siRNA. After silencing GPR30 in HCC1937 cells, P-Akt expression level was measured by Western blot analysis. GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

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