Journal of Cancer Prevention

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Fig. 5.

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Fig. 5. Effects of ethanol extracts of Scutellaria baicalensis roots (EESB) on the expression of Bcl-2 family proteins and values of mitochondrial membrane potential (MMP) in U937 cells. (A) Cells were treated with EESB for 24 hours, and aliquots containing the total protein levels were subjected to SDS PAGE followed by immunoblot analysis with the specific antibodies. (B, C) To quantify the degree of MMP loss, untreated control and EESB-treated cells were incubated with 10 μM JC-1 for 20 minutes at 37°C in the dark. The cells were then washed once with PBS, and MMP loss was evaluated using a flow cytometer. Data are presented as the mean ± SD values obtained from three independent experiments. *P < 0.05 compared with the untreated control group.
J Cancer Prev 2019;24:11~19
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