Journal of Cancer Prevention

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Fig. 4.

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Fig. 4. Activation of caspases and degradation of caspase substrates by ethanol extracts of Scutellaria baicalensis roots (EESB) in U937 cells. (A) Cells grown under the same conditions (shown in ) were lysed, and cellular proteins were visualized using the indicated antibodies and an enhanced chemiluminescence detection system. Actin was used as an internal control. (B) Cells were lysed, and aliquots (50 μg protein) were assayed for in vitro caspase-3, -8, and -9 activity using DEVD-p-nitroaniline (pNA), IETD-pNA, and LEHD-pNA as substrates, respectively, at 37°C for 1 hour. The released fluorescent products were analyzed. Data are presented as mean ± SD values obtained from three independent experiments. *P < 0.05 compared with the untreated control group.
J Cancer Prev 2019;24:11~19
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