Journal of Cancer Prevention

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Fig. 4. Inhibition of the lipopolysaccharide (LPS)-induced NF-κB activation by diallyl trisulfide (DATS) in BV2 microglial cells. The cells were pre-treated with 150 μM DATS for 1 hour before 100 ng/mL LPS treatment for 1 hour. The nuclear and cytosolic proteins were prepared for Western blot analysis using anti-NF-κB p65 and anti-IκBα antibodies and an enhanced chemiluminescence detection system. Nucleolin and actin were used as the internal controls for the nuclear and cytosolic fractions, respectively.
Journal of Cancer Prevention 2018;23:134~140
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