Fig. 3. Inhibitory effects of curcumin on dextran sulfate sodium (DSS)- induced DNA binding of NF-κB in mouse colon. The nuclear extracts was prepared from colon of each treatment group. Inhibitory effects of curcumin on DSS-induced NF-κB DNA binding were assessed by electrophoretic mobility shift assay as described in Materials and Methods. To ensure the specific NF-κB DNA binding, a competition assay was performed with 100-fold excess unlabeled oligonucleotides. Data are means ± SD (n = 5 per group). ***P < 0.001 vs. the DSS-treated group.