Journal of Cancer Prevention

eISSN 2288-3657
pISSN 2288-3649
Fig. 4.

Download original image

Fig. 4. The flow cytometric analysis for propidium iodide-based cell viability determination of HeLa cells. HeLa cells (2 × 105 cells/dish) were treated with (A) dimethyl sulfoxide (DMSO), (B) doxorubicin (DOX) (25 nM), (C) DOX (25 nM) + SCR-7 (50 μM), or (D) cisplatin (33 μM) for 48 hours and stained with propidium iodide (PI). PI-stained cells were suspended in BD staining buffer and analyzed on a flow cytometer. A minimum of 10,000 events were collected and analyzed using a 488 nm laser and 610 LP, 616/23 BP emission filters. Data is presented in scattered plot with side-scattered light (SSC) on Y-axis and PI-stained HeLa cells on X-axis. (E) Data were represented as a percentage of control and the mean ± SD. *P < 0.05, significantly different from DMSO control.
Journal of Cancer Prevention 2017;22:47~54 https://doi.org/10.15430/JCP.2017.22.1.47
© J Cancer Prev
© Korean Society of Cancer Prevention. / Powered by INFOrang Co., Ltd