Journal of Cancer Prevention

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Fig. 2.

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Fig. 2. Cytotoxic effects of DOX alone or in combination with SCR-7 in HeLa cells. HeLa cells (2 × 105 cells/well) were allowed to grow for 16 to 18 hours and were then treated with dimethyl sulfoxide (DMSO) control, DOX (25 nM), DOX (25 nM) + SCR-7 (50 μM) for 48 hours. The cells were collected, washed, and treated with 0.25% trypsin/EDTA. Cell counting with hemocytometer was performed in cells treated with trypan blue dye. (A) Before treatment with trypan blue dye, a microscope photograph was captured using a light inverted microscope (10×) and (B) Trypan blue dye exclusion-based hemocytometer counting was performed to determine the total HeLa cell number. (C) The microscopy photograph was taken at 10× after staining with trypan blue dye. (D) Trypan blue stained cells were counted with a hemocytometer to assess the presence of dead cells due to drug/inhibitor treatment. Data are presented as the percentage of trypan blue stained dead cells in each treatment condition over DMSO treatment and are represented as the mean ± SD. Each experiment was independently conducted three times. *P < 0.05 and **P < 0.01, significantly different from DMSO control.
Journal of Cancer Prevention 2017;22:47~54 https://doi.org/10.15430/JCP.2017.22.1.47
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