Journal of Cancer Prevention

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pISSN 2288-3649
Fig. 4.

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Fig. 4. Apoptosis induction and apoptosis-related protein modulation by ethanol extract of Sorbus rufopilosa (EESR) in HT29 cells. (A) 4,6-diamidino-2-phenylindole (DAPI) staining. EESR-treated cells were fixed, permeabilized, and stained with DAPI for 20 minutes. Stained cells were observed by fluorescence microscopic analysis and imaged using Axio Vision program. Arrows indicate the apoptotic bodies. Scale bar, 50 μm. (B, C) HT29 cells were treated with various concentrations of EESR for 48 hours. The cells were lysed and cellular proteins were then separated by SDS-PAGE, followed by Western blot analysis using antibodies against apoptosis-related proteins (B: p53, Fas, Fas-associated protein with death domain [FADD], Bax, and Bcl-2; C: caspase-8, caspase-9, caspase-3, and PARP). Actin was used as an internal control.
Journal of Cancer Prevention 2016;21:249~256 https://doi.org/10.15430/JCP.2016.21.4.249
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