Journal of Cancer Prevention

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Fig. 3.

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Fig. 3. Modulation of G2/M checkpoint-related protein expression in HT29 cells by ethanol extract of Sorbus rufopilosa (EESR). HT29 cells were treated with various concentrations of EESR for 48 hours. The cells were lysed and cellular proteins were then separated by SDS-PAGE, followed by Western blot analysis using antibodies against G2/M checkpoint-related proteins (A: p53, p21, cyclin A, and cyclin B; B: CHK2, p-CHK2, Cdc25C, p-Cdc25C, Wee1, cyclin-dependent kinase 1 [CDK1], and p-CDK1). Actin was used as an internal control.
Journal of Cancer Prevention 2016;21:249~256
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