Fig. 1. Effect of withaferin A on the proliferation of human colorectal cancer (HCT116) cells. (A) The structure of withaferin-A. (B) Cell viability was analyzed using MTT cell proliferation assay kits. The proliferation of HCT116 cells treated with withaferin-A was significantly decreased in a concentration-dependent manner. (C) Cell proliferation pattern of HCT116 cells was measured by counting cells after exposure to 1 μM withaferin-A during 8 days. At the last day of exposure, cells treated with withaferin-A (1 μM) showed a dramatic decrease in cell proliferation when compared with the vehicle group. All data are presented as the means ± SE; t-test and ANOVA. *P < 0.05 and **P < 0.01.

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