Journal of Cancer Prevention

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pISSN 2288-3649
Fig. 1.

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Fig. 1. Effects of carnosic acid (CA) on adipocyte differentiation, triglyceride (TG) content, and glycerol 3-phosphate dehydrogenase (GPDH) activity in 3T3-L1 cells. Preadipocytes were grown to confluency (day 0) and induced to differentiate with an optimized adipocyte differentiation medium in the presence of CA (0.1, 1, and 10 μM) throughout differentiation. (A) Morphological observation and Oil Red O staining of 3T3-L1 cells. After differentiation (day 8), the cells were fixed and stained with Oil Red O and photographed using a microscope (× 200). (B) Quantification of intracellular Oil Red O staining using spectrophotometry. (C) Quantification of TG content in 3T3-L1 cells. (D) GPDH activity in 3T3-L1 cells. Data are expressed as mean ± standard deviation (n = 4). a,b,cMeans with the different letters are significantly different from each other (P < 0.05) using Duncan’s multiple-range test.
Journal of Cancer Prevention 2015;20:41~49
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