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Journal of Cancer Prevention

Original Article

Journal of Cancer Prevention 2018; 23(3): 141-146

Published online September 30, 2018

https://doi.org/10.15430/JCP.2018.23.3.141

© Korean Society of Cancer Prevention

Proteases are Modulated by Fascin in Oral Cancer Invasion

Min Kyeong Lee, Ji Hyeon Park, Seol Hwa Gi, and Young Sun Hwang

Department of Dental Hygiene, College of Health Science, Eulji University, Seongnam, Korea

Correspondence to :
Young Sun Hwang, Department of Dental Hygiene, College of Health Science, Eulji University, 553 Sanseong-daero, Sujeong-gu, Seongnam 13135, Korea, Tel: +82-31-740-7493, Fax: +82-31-740-7352, E-mail: kiteys@eulji.ac.kr, ORCID: Young Sun Hwang, https://orcid.org/0000-0001-7012-3434

Received: August 31, 2018; Revised: September 13, 2018; Accepted: September 19, 2018

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Cancer invasion is a critical factor for survival and prognosis of patients with cancer. Identifying and targeting factors that influence cancer invasion are an important strategy to overcome cancer. In this study, we investigated the role of fascin known to be associated with cancer invasion.

Methods

Fascin depletion was performed with lentiviral short hairpin RNA against fascin mRNA and stable cell line (Fascindep) was established. Matrigel-Transwell invasion and three-dimensional (3D) culture system were used to observe fascin depletion effects. In order to observe the changes of protease secretion by fascin depleted cancer cells, protease antibody array was performed.

Results

Fascin was highly expressed in invasive cancer cells. Fascin-depleted cells showed decreased cancer invasion in Matrigel-Transwell invasion and 3D culture system. In addition, inhibition of proteases secreation and decrease of intracellular proteases mRNA expression were observed in fascin deplete cells.

Conclusions

These results indicates that fascin is closely involved in proteases activity and cancer invasion. Therefore, fascin is a strategically important factor for controlling cancer invasion.

Keywords: Metalloproteases, Fascin, Head and neck neoplasms, Gene silencing, Tumor microenvironment

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