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Cytotoxicity and Apoptosis-induced Activity of Extracts from Tetrapanax papyriferus
Cancer prevention research 2010;15:241-7
Published online September 30, 2010
© 2010 Korean Society of Cancer Prevention.

Seong-Hee Moon, Jeung-Min Lee, Hyun-Jung Kim, Youngkyoung Kang and Hae-Ryong Park
Abstract
In this study, we used methanolic extracts of Tetrapanax papyriferus (TPM) to determine their anticancer activities on HT-29 human colon cancer cells. After TPM treatment, morphological changes, such as chromatin condensation, were observed in HT-29 cells. We confirmed cytotoxicity through cell biological assays after treatment with TPM at various concentrations. An MTT reduction assay revealed that TPM treatment at a concentration of 500 Ռg/ml yielded a cell viability of 39.4%. The findings indicated that strong anticancer activity restricted colony formation at TPM concentration greater than 10 Ռg/ml. In addition, we performed apoptosis in a series of experiments in order to determine the cell biological mechanism of TPM cytotoxicity. By Hoechst staining, we confirmed the formation of apoptotic bodies in cells treated with TPM. The results of flow cytometric analysis indicated that while the number of cells in the sub-G1 phase was 0.7% in the control group and 13.1% in the group treated with 250 Ռg/ml TPM, we could identify a high apoptotic effect. Based on these results, we could confirm that TPM induces apoptosis in cancer cells and has strong anticancer properties. (Cancer Prev Res 15, 241-247, 2010)
Keywords : Tetrapanax papyriferus, HT-29 cells, MTT reduction assay, Cytotoxicity, Apoptosis

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