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Proteases are Modulated by Fascin in Oral Cancer Invasion
Journal of Cancer Prevention 2018;23:141-6
Published online September 30, 2018
© 2018 Korean Society of Cancer Prevention.

Min Kyeong Lee, Ji Hyeon Park, Seol Hwa Gi, Young Sun Hwang

Department of Dental Hygiene, College of Health Science, Eulji University, Seongnam, Korea
Correspondence to: Young Sun Hwang
Department of Dental Hygiene, College of Health Science, Eulji University, 553 Sanseong-daero, Sujeong-gu, Seongnam 13135, Korea
Tel: +82-31-740-7493, Fax: +82-31-740-7352, E-mail: kiteys@eulji.ac.kr
ORCID: Young Sun Hwang, https://orcid.org/0000-0001-7012-3434
Received August 31, 2018; Revised September 13, 2018; Accepted September 19, 2018.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: Cancer invasion is a critical factor for survival and prognosis of patients with cancer. Identifying and targeting factors that influence cancer invasion are an important strategy to overcome cancer. In this study, we investigated the role of fascin known to be associated with cancer invasion.
Methods: Fascin depletion was performed with lentiviral short hairpin RNA against fascin mRNA and stable cell line (Fascindep) was established. Matrigel-Transwell invasion and three-dimensional (3D) culture system were used to observe fascin depletion effects. In order to observe the changes of protease secretion by fascin depleted cancer cells, protease antibody array was performed.
Results: Fascin was highly expressed in invasive cancer cells. Fascin-depleted cells showed decreased cancer invasion in Matrigel-Transwell invasion and 3D culture system. In addition, inhibition of proteases secreation and decrease of intracellular proteases mRNA expression were observed in fascin deplete cells.
Conclusions: These results indicates that fascin is closely involved in proteases activity and cancer invasion. Therefore, fascin is a strategically important factor for controlling cancer invasion.
Keywords : Metalloproteases, Fascin, Head and neck neoplasms, Gene silencing, Tumor microenvironment


September 2018, 23 (3)
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