Journal of Cancer Prevention : pISSN 2288-3649 / eISSN 2288-3657

Fig. 4.

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Fig. 4. Effect of BC on DNA methylation in CD133+CD44+ HCT116 cells. Relative mRNA levels of DNMT1 (A) and DNMT3A (B) were analyzed by qPCR as described in Materials and Methods. GAPDH was detected as a loading control. (C) Global DNA methylation levels were determined in ELISAs. The AZA treatment group was used as a positive control. All analyses were conducted at least three independent experiments. One-way ANOVA and the Newman–Keuls multiple comparison test were performed. CTRL, control; AZA, 1 μM 5-aza-dC; BC, beta-carotene; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. aP < 0.05 compared to 5-aza-dC group; bP < 0.05 compared to the control group; cP < 0.01 compared to the control group.
Journal of Cancer Prevention 2019;24:224-32
© 2019 Journal of Cancer Prevention