Journal of Cancer Prevention : pISSN 2288-3649 / eISSN 2288-3657

Fig. 1.

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Fig. 1. Effects of BC on the proliferation and sphere formation capacity of CD133+CD44+ HCT116 cells. CD133+CD44+ double-stained HCT116 cells were treated with 20 μM and 40 μM BC for 6 days. (A) Cell proliferation was examined by the MTT assays as described in Materials and Methods. (B) The trypan blue exclusion assay determined the percentage of live cells after BC treatment. (C) Spheres formation was photographed under a phase contrast microscopy (× 100 magnification). (D) The relative numbers of spheres formed were counted. All data are presented as the means ± SEM. Subscripts on top of each bar indicate significant differences of group means (P < 0.05). CTRL, control; BC, beta-carotene. aP < 0.001 compared to the control group; bP < 0.05 compared to the control group.
Journal of Cancer Prevention 2019;24:224-32
© 2019 Journal of Cancer Prevention