Journal of Cancer Prevention : pISSN 2288-3649 / eISSN 2288-3657

Fig. 2.

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Fig. 2. Downregulation of AXL expression by yuanhuadine (YD) in H1299 cell line. (A) The chemical structure of YD. (B) The effect of YD on cell proliferation in H1299 cells. The cells were treated with YD for 48 or 72 hours, and the cell growth was determined by the sulforhodamine B assay. (C) The effect of YD on the expression of AXL in H1299 cells. The cells were treated with the indicated concentrations of YD for 6 hours, and cell lysates were analyzed by Western blotting. β-actin was used as a loading control. (D) The effect of YD on the expression of AXL mRNA in H1299 cells. The mRNA expression was determined by real-time PCR, and β-actin mRNA levels were used for normalization. The data are presented as the mean ± SD. **P < 0.01 by t-test.
Journal of Cancer Prevention 2019;24:217-23 https://doi.org/10.15430/JCP.2019.24.4.217
© 2019 Journal of Cancer Prevention