Journal of Cancer Prevention : pISSN 2288-3649 / eISSN 2288-3657

Fig. 3.

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Fig. 3. Inhibitory effects of genistein on migration and colony formation of MDA-MB-231 and HCC1937 cells. (A) The cells were seeded into two chambers of an insert (1.5 × 104 per well for MDA-MB-231 and 2 × 104 per well for HCC1937 cells). After 24 hours of incubation, the insert was removed, and the cells were treated with 50 μM genistein. After additional 24 hours of incubation, the gap was measured under a microscope. (B) The same procedure was repeated for scrambled siRNA and BRCA1-siRNA transfected MDA-MB-231 cells. (C) Cells were seeded in a 6-well plate at a density of 5 × 102 cells per well. The cells were then incubated for a week followed by treatment with 10 or 50 μM genistein. After additional one week of incubation, the cells were stained with crystal violet for visualization under a microscope.
Journal of Cancer Prevention 2019;24:197-207
© 2019 Journal of Cancer Prevention