Journal of Cancer Prevention : pISSN 2288-3649 / eISSN 2288-3657

Fig. 5.

Download original image
Fig. 5.

Effect of HO-1 on 15d-PGJ2-induced 15-PGDH expression. (A) HCT116 cells were treated with indicated concentrations of 15d-PGJ2 for 24 h. Total protein isolated from cell lysates was subjected to immunoblot analysis for the measurement of HO-1. (B) The mRNA level of HO-1 was determined by quantitative RT-PCR. The mRNA level of GAPDH was used as an internal control. (C) The effect of the HO-1 inhibitor ZnPP on the induction of 15-PGDH was assessed by Western blot analysis. HCT116 cells were pretreated with ZnPP (0, 5 10 μM) for 12 h. After changing the medium with a fresh one, cells were exposed to 15d-PGJ2 (10 μM) for additional 24 h. (D) HCT116 cells were treated with ZnPP (0, 1, 5 or 10 μM) for 24 h. Expression levels of 15-PGDH and COX-2 protein were determined by Western blot analysis. The data are expressed as the mean ± SD (n =3). Significant differences between the compared groups are indicated (*p < 0.05).

J Cancer Prev 2019;24:183-91 https://doi.org/10.15430/JCP.2019.24.3.183
© 2019 J Cancer Prev