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Fig. 4.

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Fig. 4.

15d-PGJ2-induced expression of 15-PGDH through activation of Akt signaling. (A) The time-related activation of Akt was assessed by measuring the phosphorylated form of Akt. HCT116 cells were treated with 10 μM 15d-PGJ2 for the indicated time periods followed by immunoblot analysis with specific antibodies. (B) The effect of the Akt inhibitor on the induction of 15-PGDH was assessed by Western blot analysis in HCT116 cells. HCT116 cells were exposed to 10 μM 15d-PGJ2 for 24 h in the presence of 0, 5 or 20 μM LY294002. Whole-cell lysates were subjected to Western blot analysis with the indicated antibodies.

J Cancer Prev 2019;24:183-91 https://doi.org/10.15430/JCP.2019.24.3.183
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