Journal of Cancer Prevention : pISSN 2288-3649 / eISSN 2288-3657

Fig. 2.

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Fig. 2.

15d-PGJ2-induced expression and catalytic activity of 15-PGDH in HCT116 cells. HCT116 cells were treated with 5, 10 or 15 μM of 15d-PGJ2 for 24 h (A) and with 10 μM 15d-PGJ2 for 1, 3, 6, 12, 18, and 24 h (B). Total protein isolated from cell lysates was subjected to immunoblot analysis for the measurement of 15-PGDH. (C) The mRNA level of 15-PGDH was determined by quantitative RT-PCR. The mRNA level of GAPDH was used as an internal control. (D) HCT116 cells were treated with 15d-PGJ2 (0, 5, 10 or 15 μM) for 24 h. By measuring the transfer of tritium from titiated PGE2 to glutamate, the activity of 15-PGDH was determined. The data are expressed as the mean ± SD (n = 3). Significant differences between the compared groups are indicated (*P < 0.05).

J Cancer Prev 2019;24:183-91 https://doi.org/10.15430/JCP.2019.24.3.183
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