Journal of Cancer Prevention : pISSN 2288-3649 / eISSN 2288-3657

Fig. 6.

Download original image
Fig. 6.

The effects of combined treatment with hispolon and TNF-related apoptosis-inducing ligand (TRAIL) on apoptosis in other renal carcinoma and normal cells. (A, B) Renal carcinoma (A498 and ACHN) cells were treated with 50 ng/mL TRAIL in the presence or absence of 50 μM hispolon for 24 hours. The level of apoptosis was measured by the sub-G1 fraction using flow cytometry. The protein expression levels of PARP and actin were determined by Western blotting. The level of actin was used as a loading control. (C, D) Caki, human skin fibroblast, and transformed mouse kidney cells (TCMK-1) cells were treated with 50 ng/mL TRAIL in the presence or absence of 50 μM hispolon for 24 hours. The cell morphology was examined using interference light microscopy (C). The level of apoptosis was analyzed by measuring the sub G1 fraction by flow cytometry (D). The values in panel A and D represent the mean ± SD from three independent samples. aP < 0.01 compared to the control.

J Cancer Prev 2019;24:155-62 https://doi.org/10.15430/JCP.2019.24.3.155
© 2019 J Cancer Prev