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Fig. 5.

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Fig. 5.

Hispolon induced up-regulation of DR4/5 expression at the post-translational levels. (A, B) Caki cells were treated with the indicated concentrations of hispolon for the indicated time periods. The protein and mRNA expression levels of DR4, DR5, and actin were determined by Western blotting and reverse transcription-PCR, respectively. The level of actin was used as a loading control. (C) Caki cells were treated with or without 50 μM hispolon in the presence of cyclohexamide (CHX) (20 μg/mL) for the indicated time periods. The protein expression levels of DR4, DR5, and/or actin were determined by Western blotting. The level of actin was used as a loading control. The band intensities of DR4 and DR5 protein were measured using the public domain JAVA image-processing program ImageJ. (D) Caki cells were treated with the indicated concentrations of hispolon for 24 hours. The protein levels of ubiquitin were determined by Western blotting. p.c, positive control, 0.5 μM MG132.

J Cancer Prev 2019;24:155-62 https://doi.org/10.15430/JCP.2019.24.3.155
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