Journal of Cancer Prevention : pISSN 2288-3649 / eISSN 2288-3657

Fig. 2.

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Fig. 2.

Combined treatment with hispolon and TNF-related apoptosis-inducing ligand (TRAIL) increases caspase activity. (A) Caki cells were treated with 50 ng/mL TRAIL in the presence or absence of 50 μM hispolon for 24 hours. Caspase activities were determined with colorimetric assays using caspase-3 (DEVDase) assay kits. (B) Caki cells were treated with 50 μM hispolon plus 50 ng/mL TRAIL for 24 hours in the presence or absence of 20 μM z-VAD-fmk (z-VAD). The sub-G1 fraction was measured by flow cytometry. The protein expression levels of PARP, procaspase-3, cleaved caspase-3 and actin were determined by Western blotting. The level of actin was used as a loading control. The values in panel A and B represent the mean ± SD from three independent samples. aP < 0.01 compared to the control. bP < 0.01 compared to the co-treatment of hispolon and TRAIL.

J Cancer Prev 2019;24:155-62
© 2019 J Cancer Prev