Journal of Cancer Prevention : pISSN 2288-3649 / eISSN 2288-3657

Fig. 1.

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Fig. 1.

Hispolon enhances TNF-related apoptosis-inducing ligand (TRAIL)-induced apoptosis. (A) Caki cells were treated with 40 or 50 ng/mL TRAIL in the presence or absence of the indicated concentrations of hispolon for 24 hours. The sub-G1 fraction was measured by flow cytometry as an indicator of the level of apoptosis. The protein expression levels of PARP and actin were determined by Western blotting. The level of actin was used as a loading control. (B) The cell morphology was examined using interference light microscopy. (C) The condensation and fragmentation of the nuclei were detected by 4′,6-diamidino-2-phenylindole staining. The cytoplasmic histone-associated DNA fragments were determined by a DNA fragmentation detection kit (D). The values in panel A and D represent the mean ± SD from three independent samples. aP < 0.01 compared to the control.

J Cancer Prev 2019;24:155-62
© 2019 J Cancer Prev