Journal of Cancer Prevention : pISSN 2288-3649 / eISSN 2288-3657

Fig. 5.

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Fig. 5. Involvement of extracellular signal-regulated kinase (ERK) in Z-ajoene-induced NAD(P)H:quinone oxidoreductase-1 (NQO1) expression in MCF-10A cells. (A) The effects of Z-ajoene on the phosphorylation of upstream kinases. Western blot analysis was performed to examine the expression of nuclear factor E2-related factor-2 (Nrf2) and NQO1 and the phosphorylation of ERK, PKCδ, JNK, and p38 in cells treated with 20 μM Z-ajoene. (B) Nuclear extracts from cells treated with U0126 (20 μM) for 1 hour prior to the treatment with 20 μM Z-ajoene were subjected to Western blot analysis of Nrf2. Lamin B was used as a nuclear protein marker. (C) MCF-10A cells were treated with U0126 for 1 h prior to the treatment with 20 μM Z-ajoene for 20 hours. Cell lysates were subjected to Western blot analysis to examine the expression of NQO1. (D) MCF-10A cells were incubated with N-acetyl-L-cysteine (NAC) (5 mM) for 1 hour prior to the treatment with 20 μM Z-ajoene for indicated periods. Cell lysates were subjected to Western blot analysis to examine the phosphorylation of ERK. Actin was used as a loading control.
Journal of Cancer Prevention 2019;24:112-22 https://doi.org/10.15430/JCP.2019.24.2.112
© 2019 Journal of Cancer Prevention