Journal of Cancer Prevention : pISSN 2288-3649 / eISSN 2288-3657

Fig. 4.

Download original image
Fig. 4. The effects of reactive oxygen species (ROS) by Z-ajoene on the expression of NAD(P)H:quinone oxidoreductase-1 (NQO1) and nuclear factor E2-related factor-2 (Nrf2). (A, B) MCF-10A cells were incubated with N-acetyl-L-cysteine (NAC) (5 mM) for 1 hour prior to the treatment with Z-ajoene at indicated concentrations for 6 hours (for measuring ROS) or 20 hours (for Western blot analysis). (C, D) Possible role of thiol modification in Z-ajoene-induced expression of NQO1. MCF-10A cells were incubated with a thiol reducing agent dithiothreitol (DTT) (0.5 mM) for 1 hour prior to treatment with Z-ajoene (20 μM) for 6 hours (for measuring ROS) or 20 hours (for Western blot analysis). (A, C) The ROS generation was measured by 2′,7′-dichlorofluorescein diacetate assay followed by flow cytometry. Bar graphs show relative numerical values of ROS-positive cells (mean values). Error bars represent means ± SD of three independent experiments. Asterisks indicate the statistically significant difference as compared with control (*P < 0.05; **P < 0.01). (B, D) Cell lysates were subjected to Western blot analysis to examine the expression of NQO1 and Nrf2. Actin was used as a loading control.
Journal of Cancer Prevention 2019;24:112-22 https://doi.org/10.15430/JCP.2019.24.2.112
© 2019 Journal of Cancer Prevention