Journal of Cancer Prevention : pISSN 2288-3649 / eISSN 2288-3657

Fig. 3.

Download original image
Fig. 3. The effects of ajoene on the reactive oxygen species (ROS) generation and intracellular glutathione (GSH) accumulation. ROS generation by Z-ajoene was compared with that by E-ajoene. (A, B) MCF-10A cells were incubated with E- or Z-ajoene (20 μM each) for 6 hours and the generation of ROS was measured by the 2′,7′-dichlorofluorescein diacetate (DCF-DA) staining followed by a fluorescence microscope (A) or flow cytometry (B-1) as described in Materials and Methods. Scale bar: 200 μm. (B-2) Bar graphs show relative numerical values of ROS-positive cells (mean values). Error bars represent means ± SD of three independent experiments. Asterisks indicate the statistically significant difference as compared with control (*P < 0.05; **P < 0.01). (C) MCF-10A cells were incubated with 20 μM each of E- (C-1) or Z-ajoene (C-2) for indicated periods and the generation of ROS was measured by the DCF-DA staining followed flow cytometry. (C-3) Bar graphs show relative numerical values of ROS generation by E-ajoene (white columns) or Z-ajoene (black columns). Error bars represent means ± SD of three independent experiments. Asterisks indicate the statistically significant difference as compared with control (*P < 0.05; **P < 0.01; ***P < 0.001). (D) Changes in total intracellular GSH levels in response to Z-ajoene treatment in MCF-10A cells. The non-toxic concentration (20 μM) of Z-ajoene was treated to culture medium for indicated time periods, and total cellular GSH content was measured as described in Materials and Methods. Results are presented as the fold induction of intracellular GSH levels in Z-ajoene-treated cells compared with that in the control cells. Error bars represent means ± SD of three independent experiments. ***Significantly different from the untreated control (P < 0.001).
Journal of Cancer Prevention 2019;24:112-22 https://doi.org/10.15430/JCP.2019.24.2.112
© 2019 Journal of Cancer Prevention