Journal of Cancer Prevention : pISSN 2288-3649 / eISSN 2288-3657

Fig. 1.

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Fig. 1. The molecular structures and the effects of ajoene on the antioxidant protein expression. (A) Naturally occurring ajoene isomers in garlic extracts. (B) MCF-10A cells were incubated with E- or Z-ajoene (20 μM each) for indicated periods. Whole cell lysates (40 μg protein) were separated by 12% SDS-PAGE and immunoblotted with antibodies of NAD(P)H:quinone oxidoreductase-1 (NQO1) and actin. Actin was measured to ensure equal protein loading. (C) MCF-10A cells were incubated with Z-ajoene at indicated concentrations for 20 hours. Whole cell lysates were performed SDS-PAGE and immunoblotted with antibodies of NQO1, heme oxygenase-1 (HO-1), glutamate–cysteine ligase catalytic subunit (GCLC), and nuclear factor E2-related factor-2 (Nrf2). Actin was used as the loading control. The band intensity of immunoblots for NQO1, HO-1, and GCLC was normalized to that of actin by GelPro image densitometry followed by statistical analysis. Bar graphs show relative numerical values of the band intensity of NQO1 (white columns), HO-1 (gray columns), or GCLC (black columns). Error bars represent means ± SD of three independent experiments. Asterisks indicate the statistically significant difference as compared with control (*P < 0.05; **P < 0.01; ***P < 0.001).
Journal of Cancer Prevention 2019;24:112-22
© 2019 Journal of Cancer Prevention