Journal of Cancer Prevention

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Fig. 2. Effect of fascin depletion on proteases activity. (A) Released protease in Mock and Fascindep cells. Conditioned media from Mock and Fascindep cells was incubated with Protease Array Kit and relative signal intensities were determined using ImageJ software program. Results were plotted. aP< 0.01 versus Mock. Proteases with large signal changes are indicated by circled numbers. (B) Expression of proteases mRNA in Mock and Fascindep cells. Reverse transcription-PCR products were loaded into agarose gel and glyceraldehyde-3-phosphate dehydrogen-ase (GAPDH) was used as RNA loading control. Results were plotted. bP < 0.05, aP < 0.01 versus Mock. MMP, matrix metalloproteinase.
Journal of Cancer Prevention 2018;23:141~146
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