Primer sequences, product sizes, and annealing temperatures for reverse transcription-PCR
Target gene | Primer sequence | Annealing temperature (°C) |
---|---|---|
MMP-9 | Sense; 5′-GATGCGTGGAGAGTCGAAAT-3′ | 65 |
MMP-10 | Sense; 5′-GACCCCAGACAAATGTGATCCT-3′ | 60 |
Cathepsin B | Sense; 5′-GAAGAAGCTGTGTGGCACTG-3′ | 58 |
Cathepsin D | Sense; 5′-AGGTGAAGGAGCTGCAGAAG-3′ | 58 |
Kallikrein 5 | Sense; 5′-CCCCAAGTGCACTTCCCTAA-3′ | 60 |
GAPDH | Sense; 5′-ATCCCTCCAAAATCAAGTGGG-3′ | 60 |
MMP, matrix metalloproteinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.