JCP

eISSN 2288-3657
pISSN 2288-3649

Table 1

Primer sequences, product sizes, and annealing temperatures for reverse transcription-PCR

Target gene	Primer sequence	Annealing temperature (°C)
MMP-9	Sense; 5′-GATGCGTGGAGAGTCGAAAT-3′Antisense; 5′-CACCAAACTGGATGACGATG-3′	65
MMP-10	Sense; 5′-GACCCCAGACAAATGTGATCCT-3′Antisense; 5′-TTCAGGCTCGGGATTCCA-3′	60
Cathepsin B	Sense; 5′-GAAGAAGCTGTGTGGCACTG-3′Antisense; 5′-GTTCGGTCAGAAATGGCTTC-3′	58
Cathepsin D	Sense; 5′-AGGTGAAGGAGCTGCAGAAG-3′Antisense; 5′-ATTCCCATGAAGCCACTCAG-3′	58
Kallikrein 5	Sense; 5′-CCCCAAGTGCACTTCCCTAA-3′Antisense; 5′-TCGTGTAGACACCCGGTCTG-3′	60
GAPDH	Sense; 5′-ATCCCTCCAAAATCAAGTGGG-3′Antisense; 5′-TGAAGACGCCAGTGGACTCC-3′	60

MMP, matrix metalloproteinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

Journal of Cancer Prevention 2018;23:141~146 https://doi.org/10.15430/JCP.2018.23.3.141