Journal of Cancer Prevention

eISSN 2288-3657
pISSN 2288-3649

Primer sequences, product sizes, and annealing temperatures for reverse transcription-PCR

Target genePrimer sequenceAnnealing temperature (°C)
MMP-9Sense; 5′-GATGCGTGGAGAGTCGAAAT-3′Antisense; 5′-CACCAAACTGGATGACGATG-3′65
MMP-10Sense; 5′-GACCCCAGACAAATGTGATCCT-3′Antisense; 5′-TTCAGGCTCGGGATTCCA-3′60
Cathepsin BSense; 5′-GAAGAAGCTGTGTGGCACTG-3′Antisense; 5′-GTTCGGTCAGAAATGGCTTC-3′58
Cathepsin DSense; 5′-AGGTGAAGGAGCTGCAGAAG-3′Antisense; 5′-ATTCCCATGAAGCCACTCAG-3′58
Kallikrein 5Sense; 5′-CCCCAAGTGCACTTCCCTAA-3′Antisense; 5′-TCGTGTAGACACCCGGTCTG-3′60
GAPDHSense; 5′-ATCCCTCCAAAATCAAGTGGG-3′Antisense; 5′-TGAAGACGCCAGTGGACTCC-3′60

MMP, matrix metalloproteinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

Journal of Cancer Prevention 2018;23:141~146 https://doi.org/10.15430/JCP.2018.23.3.141
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